Karl D. Gordon University of Arizona Spitzer Observations of the Aromatic Features in M101 HII Regions and Starbursts I will discuss the study of the Aromatic dust emission features in M101 HII regions and a large sample of starburst galaxies using the Spitzer Space Telescope. M101 is unique in that it is face-on and has large metallicity gradient ranging from log(O/H) of ~8.8 in the nucleus to 7.5 at its edge. The sample of 44 starburst galaxies was picked to have metallicities ranging from the lowest known (i.e., I Zw 18) to super solar (7.2 < 12+log(0/H) < 9.3). Spitzer spectroscopy and imaging is used to probe the behavior of the Aromatic emission features (also commonly called the PAH features) as a function of HII region diagnostics. The Aromatics are seen to decrease in strength with decreasing metallicity (increasing excitation) in both M101 HII regions and starburst galaxies. In addition, the feature strength ratios change systematically from the high metallicity, low excitation regions to the low metallicity, high excitation regions. The feature variations at face value do not follow the predictions of the PAH model (neutral versus ionized PAH molecules) and may be more consistent with a solid state carrier (e.g., Hydrogenated Amorphous Carbon, Quenched Carbonaceous Composite, coal, etc.). Thus, studies of the aromatic features in star forming regions give us important clues to identifying their carrier which, in turn, helps to more accurately model these features in studies ranging from objects in the Milky Way to number counts in deep infrared surveys.